Public speaking Assignment Example | Topics and Well Written Essays - 250 words - 2

Open talking - Assignment Example She pointed that the administration could accomplish something of the sort to acknowledge all the mistreated individuals. The assembly hall acclaimed when she completed yet they didn’t hear her out answer and praised simply after the final words ‘Statue of Liberty’. The criticism of the crowd wasn’t called by the center of her discourse, however by the way that need to some way or another respond. The adverse input of the adversary of the primary character depended on the individual threatening vibe and shouldn’t be taken as a valuable pundit. Talking about the principle character’s reaction to the opponent’s hostility, we should make reference to that as a contention professional her words that it was the 50th birthday celebration. It wasn’t, really, a contention or even a significant truth. Along these lines, being in a similar circumstance I would feel very apprehensive and irritated and respond another way, for instance - att empt to give another contention. The young ladies began contending on the field of their shared abhorrence and gave the implausible contentions. So we can infer that the input of the crowd and the adversary wasn’t objective at

societhf Rejection of Civilization in The Adventur Essay Example For Students

societhf Rejection of Civilization in The Adventur Essay societhfRejection of Civilization in The Adventures of Huckleberry Finn Adventures Huckleberry Huck Finn EssaysRejection of Civilization in The Adventures of Huckleberry Finn In the novel Huckleberry Finn by Mark Twain, Huck chooses to dismiss human advancement. Toward the finish of the story Aunt Sally needs to socialize him, yet he won't. He says I figure I got the opportunity to light out for the region in front of the rest, since Aunt Sally, shes going to embrace me acculturate me, and I cannot stand it. Ive been there previously. Huck chooses to pick against society due to all the brutal real factors that he has seen direct. Hucks early questions of the cultivated world all began with Pap. During the vast majority of his adolescence, Huck had been manhandled both genuinely and intellectually by his redneck watchman Pap. This man had strolled into and out of Hucks life on various events. He was the main dad figure in Hucks life and bombed wretchedly at the specific employment. Pap was the main portrayal of human progress to Huck and it was a harsh one. It was additionally human progress that granted authority of Huck to Pap. He had been cheated too often by the enlightened world, and that was the primary explanation he chose to venture out from home. Huck ran from his difficulties at home down the Mississippi River. The stream is the place he discovered his asylum. Jim and Huck were consistently sheltered, autonomous, and free out on the pontoon. It appeared that each time they would go to shore, something negative including progress would emerge. The clouded side of human instinct and enduring would get together with both of them. They generally discovered the under-paunch of society. The image of human enduring was the Grangerfords family. At the point when Huck wound up before their ranch after the boat wreck, his initial introduction was a positive one. He imagined that the Grangerfords were a lovely, ordinary family. Anyway the dull insider facts that existed inside the family could cause hair to stand on end. The compositions and works made by Emmeline Grangerford, who passed on when she was fourteen, are of rather dismal subjects. She was a destroyed youngster that originated from an unusual, upset family. They had a primitive war going with another family where steady passings and enduring occurred. Not long before Huck leaves, his age identical and companion Buck, gets shot without a second thought. Simply one more praiseworthy execution of this alleged progress that Huck should come back to. The ruler and duke anyway were the what tops off an already good thing. They spoke to the voracity and extortion that Huck particularly loathes about society. From the second that Huck and Jim got together with the ruler and duke, the journey got ugly. These two rascals were hoodlums and killers, yet they likewise wound up selling Jim as a runaway slave. From that point onward, the two attempted to cheat several guiltless young ladies out of their family fortune. This hanase demonstration is the thing that caused Huck to feel the most noticeably terrible about individuals by and large. The statement It was sufficient to make a body embarrassed about humankind, (162) demonstrated that Huck was humiliated to be a human. He would never return to a development which he had no regard for. As per Huck, the ethics of society were up the rivulet. Huckleberry Finn was managed a terrible arrangement throughout everyday life. It began with his damaging dad Pap. Strength is the most significant part of youth and Huck didnt have that. Pap continued leaving and returning into his life and even went as low as to endeavor to take Hucks cash. He chooses to flee and make his journey down the waterway. .ucab08a2fd5c21a7b2c7dfda7ff56c40c , .ucab08a2fd5c21a7b2c7dfda7ff56c40c .postImageUrl , .ucab08a2fd5c21a7b2c7dfda7ff56c40c .focused content territory { min-tallness: 80px; position: relative; } .ucab08a2fd5c21a7b2c7dfda7ff56c40c , .ucab08a2fd5c21a7b2c7dfda7ff56c40c:hover , .ucab08a2fd5c21a7b2c7dfda7ff56c40c:visited , .ucab08a2fd5c21a7b2c7dfda7ff56c40c:active { border:0!important; } .ucab08a2fd5c21a7b2c7dfda7ff56c40c .clearfix:after { content: ; show: table; clear: both; } .ucab08a2fd5c21a7b2c7dfda7ff56c40c { show: square; progress: foundation shading 250ms; webkit-change: foundation shading 250ms; width: 100%; haziness: 1; change: obscurity 250ms; webkit-progress: murkiness 250ms; foundation shading: #95A5A6; } .ucab08a2fd5c21a7b2c7dfda7ff56c40c:active , .ucab08a2fd5c21a7b2c7dfda7ff56c40c:hover { darkness: 1; progress: mistiness 250ms; webkit-change: mistiness 250ms; foundation shading: #2C3E50; } .ucab08a2fd5c21a7b2c7dfda7ff56c40c .focused content zone { width: 100%; position: relat ive; } .ucab08a2fd5c21a7b2c7dfda7ff56c40c .ctaText { outskirt base: 0 strong #fff; shading: #2980B9; text dimension: 16px; textual style weight: intense; edge: 0; cushioning: 0; content enhancement: underline; } .ucab08a2fd5c21a7b2c7dfda7ff56c40c .postTitle { shading: #FFFFFF; text dimension: 16px; text style weight: 600; edge: 0; cushioning: 0; width: 100%; } .ucab08a2fd5c21a7b2c7dfda7ff56c40c .ctaButton { foundation shading: #7F8C8D!important; shading: #2980B9; fringe: none; outskirt range: 3px; box-shadow: none; text dimension: 14px; text style weight: striking; line-stature: 26px; moz-outskirt span: 3px; content adjust: focus; content beautification: none; content shadow: none; width: 80px; min-tallness: 80px; foundation: url(https://artscolumbia.org/wp-content/modules/intelly-related-posts/resources/pictures/basic arrow.png)no-rehash; position: total; right: 0; top: 0; } .ucab08a2fd5c21a7b2c7dfda7ff56c40c:hover .ctaButton { foundation shading: #34495E!important; } .ucab08a2fd5c 21a7b2c7dfda7ff56c40c .focused content { show: table; stature: 80px; cushioning left: 18px; top: 0; } .ucab08a2fd5c21a7b2c7dfda7ff56c40c-content { show: table-cell; edge: 0; cushioning: 0; cushioning right: 108px; position: relative; vertical-adjust: center; width: 100%; } .ucab08a2fd5c21a7b2c7dfda7ff56c40c:after { content: ; show: square; clear: both; } READ: The utilization of modalizers in a film survey: The Passio Essay Every time that they go to shore, some reject of human advancement is who they would run into. Auntie Sally needs him to return with her and socialize him, yet dependent on his encounters, Huck simply cant let that occur. He needs to wander aimlessly. The main thing that Pap was always unable to take from his child was his creative mind. He should have the option to think uninhibitedly

More questions

More questions Today is Registration Day at MIT, and tomorrow (Tuesday) is the first day of Spring Term! Im a little behind on the questions, so here goes though, due to my time restrictions during application season and the number of questions, my answers must be brief (sorry for the brevity). Will asks, Does anyone know if CPW is restricted to students who are accepted, or is it open to anyone who wants to go? Also, midwest mom asks, Can you please comment about the Campus Preview Weekend? My son is planning to attend, and I wonder if parents are welcome as well? Will there be any programs for parents, or is this weekend geared to convincing prospective students of the obvious merits of MIT? Campus Preview Weekend (CPW) is a four day event which is only for admitted students and their parents each April. Currently, admitted students have information about the weekend on their MIT portal; in fewer than two weeks, we will send out a brochure of information about CPW to families. There will definitely be programs for parents as well as students; parents are more than welcome at CPW! If we can help you plan for CPW in any way, please call the CPW hotline at (617) 258-6085. Nbot1 asked, Since you like Hindi music, I was wondering if you like bhangra? I love bhangra! :) Yes! Bhangra is awesome. Bhangra (energetic Punjabi/Indian folk dance) is pretty big at MIT; for more info, check out the MIT Bhangra Club and Bhangra Blast. A.M.C. asks, In that case, may I submit a few pieces of artwork (they are in .BMP format) as part of my supplemental material package? We are happy to take submissions of art, music, etc, but it is starting to get a little late in the process to have these things evaluated by our faculty before we go to committee. We will continue to accept any important update to your file until we mail decision letters. And, applicants in US school systems, be sure to get us your Mid-Year Grade Report as soon as possible! parent asked four questions. Question 1: How do you look deeper? To what depth? As it is entirely subjective, does each reader defines their own understanding of depth? Or is there a standardized procedure that all readers/admission officers at MIT follow to uncover depth? If so what is that procedure? Before each reading season, the reading staff goes through training. We read actual files, talk about what would be the elements of a good read for each file, what insights we should have seen from each file. Newer readers are overseen by my seasoned readers, who not only double check files, but also continually offer mentoring and guidance. Experience, reading lots and lots of applications, is the best way to gain insight. Question 2: Is there a standardized definition for best match to MIT? What is it? Or does each reader/admission officer at MIT have their own understanding of it? This is also part of the training. There is no one best match to MIT, but rather many flavors of best match, many of which Ive discussed in my previous post. Question 3: Does the assessment during committee process automatically lead to the magic number of students best matched with MIT to equal the available spaces? Question 4: And if there is indeed some comparison because the number of students best matched with MIT than number of available spaces, then how is such comparison done? Is there a standardized procedure to make such comparisons, what are the metrics if any for such comparisons? The committee process has many stages. We evaluate each student individually, and if we admit them if they are part of the class we want to build and a good match. Inevitably, after evaluating all of the files, we have admitted more than our target number of admitted students. So, we repeat the process, with more rigor and vigilance. Jordan asked, I know a lot of teachers include a comment somewhere in their rec letter(s) regarding contact information if there are any questions. Do you know if the AdComm has ever actually contacted a teacher or other faculty member to ask something before? I certainly have. Usually, an application file gives me the information I need, but when it doesnt, I dont hesitate to call a teacher or guidance counselor. Shahab asked, We all know that you and Ben are two of the people who are doing the application reading. What about the rest? Who else is in your team? How many people are there? Are they professors or students at MIT? The bulk of the reading is done by the professional full-time admissions staff, but students and professors do help read applications and select the class. In a future post, Ill tell you more about my colleagues, the MIT admissions officers. Jeremie L. asked, Im curious as to how you became an Ass. Director of Admissions. Well, Jeremie, if you have to abbreviate it I prefer Asst. Director of Admissions but anyway, Ill talk a little more about this in the future, and in the meantime you can read my introductory post. Recruited Athlete asked, I have a question, how much does getting recruited help you in the admissions process, if at all? Athletic talent, like music, art, theater, etc. talent, is a talent that you bring to the admissions process. For music, we have our music faculty evaluate applicants talents; for athletes, we have our varsity coaches help us with evaluation. We are proud of having one of the largest athletics programs in the nation (I believe that only Harvard, a Division I school, has a larger number of varsity sports teams). MIT is a Division III athletics school, though, so athletics arent something that will get you in the backdoor. As Marilee says, theres only one way to get into MIT, and its the hard way. expatmom asked, Im curious about the process used to evaluate international/non-U.S. school system applicants. Do you use the same numerical index for ranking the applicants academic preparation? Are international/non-U.S. school system applications read geographically? Yes, the numerical index is one guide for our process for both domestic and international applicants. We do not read domestic (US citizens and permanent residents) applicants by region. I assure you, though, that we are very familiar with international schools, and evaluate applicants from international schools in the proper context. Like Ive written, school and geography are important parts of context, and context is very important in our process. expatmom also asks, Will the status of the Mid-Year Grade Report on the application tracking site eventually indicate that this report is waived for non-U.S. school system applicants, or will it continue to indicate that the report has not been processed? I dont know the answer to this question, but watch the comments thread for this post, where hopefully Ben and/or Quinton will answer the question. Thanks for all your questions and comments! Keep commenting =)

Social Media Communication - Free Essay Example

Sample details Pages: 2 Words: 533 Downloads: 6 Date added: 2019/04/10 Category Society Essay Level High school Topics: Social Media Essay Did you like this example? Marketing is a difficult job to accomplish for most companies as they are looking to increase sales and build up goods customer relationships. Therefore, companies have come up with many innovative solutions to gain the attention of its customers. Traditional approaches to marketing are not as practical as they were in the past and hence, demand newer methods to be applied, one such way is social media marketing. With the rise of the internet, social media has become a part of the daily life for many individuals. These individuals are often the ones making sales often influencing sales decisions. Hence, it is crucial to appeal to these customers in the media of their choice. Moreover, nowadays customers are a lot more knowledgeable than they were before which makes it harder to come up with a marketing campaign that is both attention-grabbing and morally and factually correct. Social medias such as Facebook and Twitter provide advertisements based on the preferences of the user which narrows down the target audience for the brand automatically. Therefore, social media can be exceptionally helpful in promoting a brand. In my interview with the regional manager of Pepsi Co., I asked various questions related to their social media marketing strategy. Pepsi Co. was a little late in joining the social media marketing bandwagon, but ever since its entry, it has invested a lot into the company online presence. Don’t waste time! Our writers will create an original "Social Media Communication" essay for you Create order Coca-Cola and Pepsi Co. have always been the two most prominent competitors in the beverage industry. While Coca-Cola was early in capitalizing social media potential, Pepsi Co. has tried its best to come in front with Coca-Cola. However, Coca-Cola currently has over 55 million followers on Facebook while Pepsi Co. has 9 million. This is still a considerable achievement considering that in 2010, this following was only 4 million. Pepsi Co. has gained all those followers by investing in their Refresh Project. This project primarily risked a large sum of money, budgeted for the super bowl which had proven returns, on a social campaign costing $20 million in the year of 2010. Pepsi Co. was successful in combining social responsibility with social media marketing and their following on Facebook went from 25,000 to about 9 million as of today. Also, this was a massive success for them as it also increased the traffic on their website by a whopping 800%. Even when Pepsi has been known to make innovative TVCs and marketing campaign, their marketing strategies were insufficient without proper social media marketing. Their company has a large customer base which requires them to infuse more loyalty into their customers. For this, they must relate to their customers on a more personal level. Pepsi uses personalized questions and asks their followers to share their pictures with the brand to win giveaways and prizes to gain even more followers. They frequently affiliate themselves with social causes and help raise awareness to establish its image as not just a company driven by profits but also by caring and responsible individuals of the society. At Pepsi Co., the management believes that marketing is based on social relationships and with the help of social media customer can express what they like about the brand, and this word-of-mouth marketing drives their profits and revenues (Silverstein, 2010).

The Hall Of Fame For College Basketball - 1500 Words

The Hall of Fame for college basketball players was on the wall. It had just recently been updated after the Hall of Fame presentation of Saturday. I had just woken up feeling cold. I looked out the window of my dorm to see the dark, cloudy day outside with the rain pelting down on the window pane. I was still really tired after the late night of getting praised on my amazing basketball skills, finally I had been recognized and everyone was telling me that I needed to declare that I’m joining the NBA as soon as possible! I then declared for the NBA draft halfway through my season. The election for the new president was going on at that moment, but he wasn’t really interested in the elections, he heard on the radio what the soon to be president didn’t like and what he did. The main thing he heard though was that he didn’t like basketball, how could anyone not like basketball? How could I get rid of this new president to be? I looked up on the black market on the deep dark web for an assassin. There were a few ads in there for Hit man’s pest control and Hit man agent. I rang the Hit man agent and there was a muffled voice coming over the phone, I could barely understand him. I told him that I wanted the election to be stopped and the person who was winning the election to be killed, and cover up your tracks well! There was one thing I didn’t want to happen. I didn’t want to be arrested just as I was about to get into the NBA. No one would want me on their team when theyShow MoreRelatedDean Smith Essay1163 Words   |   5 Pagesmens college basketball. He is originally from Emporia, Kansas, Smith as been called a coaching legend by the Basketball Hall of Fame. Smith is best known for his successful 36-year coaching career at University of North Carolina at Chapel Hill. 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Tom Ang Free Essays

Tom Ang grew up playing the clarinet. His dream was to become the first clarinet for the London schools symphony orchestra. he never made his dream in the musical world so he turned to the world of Medicine. We will write a custom essay sample on Tom Ang or any similar topic only for you Order Now he stayed in the Nottingham medical school for 3 years before he decided that this wasn’t his passion either. he traveled to Hong Kong many years later and saw the beauty that he has never seen before. he then decided that this was his passion. to photograph the things people pass by everyday and to have a new world seen from right under our noses. He traveled to mexico to see not the poverty that is riddled throughout the land but to see the happiness that the families share even in the dark times. to show that it doesn’t matter how much money you have to care for your family but how much heart. In his trip to mexico he realized that people don’t want to always see the darker things in life that may seem interesting. he saw past the mirror that makes other people resilient and chooses to take pictures of the obvious things that others won’t see. Tom decided that these pictures are the ones that show true heart and are willing to break the boundaries of normal lifestyle and show the cultures of many other races and nations. he also visits New York in that same year and tries to show New York from the outside worlds perspective. this meaning the poverty that surrounds New York so that it seems similar to mexico and the other countries. he soon realizes that these people are not like the ones in his other photographs that these people are dangerous and are not fueled by heart and love but they are fueled by hate and greed. He tries to capture these pictures as best as he can but did not post many because he believed that they didn’t show the true feeling he felt when taking the photos and it doesn’t have the same effect if it doesn’t feel the same. At this point in time Tom started working with Fay Godwin as his assistant. he was asked if he knew how to print because that would be his basic job, and of course he responded yes. He claims that he was a total amateur and did not want to admit it because he needed the job. Yet for some reason she kept him on the team and trained him to become a better printer so he can print for her book and galleries. Tom traveled back to Hong Kong and took many more homescape photos while on his vacation there. Tom had upset a large nest of bees when photographing there. they attacked him very vigorously and sent him to the operating table. he says that his life could have ended right there. after his battle with the bees he traveled to japan in search of a dream boat that kept appearing every time that he would dream. he searched through all of japan taking pictures of boats so he could find it. but sadly this boat was never found. How to cite Tom Ang, Papers

Social influence in psychology free essay sample

Therefore, upon examination of the entities of an individuals social status can lead to the study of behavioral occurrences, like aggression and violence among children. Social influence can change an individual over time. Psychology is the cience of human and animal behavior; behavior is sometimes advert, or public (Kowalski Westen, 2009). There are many types of behavior; some patterns in lower forms described as instincts, such as a child playing with a toy. Behavior can range from simple, involuntary reflexes, and too complicated. (Bornstein et al. , 2010). Like science, finding a cause for behavior was achieved by the use of research and theories. However, research initiates with a problem that requires special attention to resolve. Aggression and Violent Behaviors Aggression is an attack or hostile action directed towards another person or thing. Aggression and violence are two forms of behavior, which are associated with one another, but do not necessarily happen at the same time. We will write a custom essay sample on Social influence in psychology or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page When an individual lashes out, this physical aggression is hostile aggression (Kowalski Westen, 2009, p. 643). Children react from violent acts or aggressive behavior, within society we live in aggression and violence play an important role in culture differences. To describe the content in which behavior is measured is variables. The second type of ggressive behavior that occurs within children is instrumental aggression, meaning where the persons behavior is pragmatic or calm (Kowalski Westen, 2009, p. 643). Influences on Behavior First, the association between violence and aggression at school among children shows awareness that exposed to violence within the school can influence a childs behavior. (Social Consequences Alone in Groups, 2010, p. 1). These groups have a social impact on a childs social behavior, when discussing violence. Having an understanding of how violence exposure related to children, may inform use of reventive interventions aimed at reducing violence. During a childs life, parents play an important role. Often parents look for aggressive behavior and not knowing if behavior is normal, action needs to be taken. Many parents look for clues or aggression and violence among their own children. If a parent that shows aggressive or violent behavior brings up a child, the child will follow the same footsteps later on in life. The behavior that a parent displays socially to their child becomes appropriate behavior. Nevertheless, a parent can increase a childs chances by eveloping this trait of behavior by commenting on the childs behavior. Negative comments will increase aggressive and violent behavior. Peer Group and Behavior All of these social behaviors are manipulable by changing within a group. When individuals gather groups, the fewer the number the fewer negative social comments. Social loafing and social facilitation have been linked in experiments in the past, but social loafing and deindividuation is not affiliated. Social loafing is a general finding that when people perform in groups but do less work or put less effort when working alone. There are keys elements linked to social loafing: the identifiability of participants, evaluation of performance within a group, difficulty of the task, and expectations (Arnett, 1996, Para. 2). Social behavior can influence a childs life. Social facilitation is which children perform better alone or within a small group. Children do better perform ing a single task, but performance worse in a larger group. If the child is subjected to feel uncomfortable, aggressive behavior can result. In recent years, social loafing and social facilitation were the same (Arnett, 996, Para. 4). Deindividuation is a finding that a group of individuals produces more deviant, aggressive, or sociable unacceptable behaviors. These variables involve children in-group behaviors, when left alone. A child will look at a group of individuals that they need acceptance from; the child will look for a way to be accepted. By modeling, this specific behavior within a group is using available references of the group, to distinguish behaviors. Some obvious precursors of aggression of children are which the child is being harassed or teased. These items rought on will influence the child to show violent behavior, and be aggressive towards other individuals. The social influence that a child receives affects behavior in many ways. The child may be finding difficulty in school, home, or with friends. The child will seek reinforcement, by looking to fit in a positive social group. Looking to help a child with behavior therapeutic intervention determines by how the parent feels if the childs behavior is normal and there is no sign of aggression. Social is the way an individual behaves, thinks, and feels. The roots of aggressive behavior lie in social rewards, punishments, and cognitive processes such as observational learning. The general model for aggression states that people interact with situations to determine the general output. The capacity for aggression and violence appears to be innate, but aggression and violence depend on learning and culture (Kowalski westen, 2009). Conclusion Human behavior when speaking of has factors that influence development of social behavior. Social behavior influence people, while biological traits aid to the ehavioral patterns.

Lab Report of the Experiment of Conjugation of E. Coli Essay Example

Lab Report of the Experiment of Conjugation of E. Coli Paper Using alkaline lysine nipper, a DNA lassie was isolated from the donor and transcontinental strains and FIG electrophoresis was used to determine the size of the plasmid. The conjugation efficiency was found to be 16. 25% and the plasmid DNA was approximately 97 kilobytes long. The results show that the F plasmid was effectively transferred from the donor cells into the recipient cells via conjugation. Introduction:Bacterial conjugation is the unidirectional transfer of either genomic DNA or plasmid DNA from a donor bacterial cell to a recipient bacterial cell by cell-to-cell contact via a sex pills (Sonatas Simmons, 2006). Conjugation was first discovered by Elderberry and Datum in 1946. In their experiment, they grew two strains of bacteria in separate vessels with rich medium and then together in one vessel containing the same medium. Then, they spread the three vessel contents onto medium agar plates and incubated them overnight at ETC. The only plate that showed cell growth was the plate containing the mixture of the two bacterial strains. The other two plates showed no growth. This experiment proved that in order for recombination to occur, the two strains must come in contact with one another (Elderberry, Datum, 1946). In 1950, Bernard Davis discovered that cell-to-cell contact was required to obtain a transcontinental. Using a U tube containing a sintered filter between the two sides of the tube, he added two types of bacteria (donor and recipient) to each side of the tube. Because of the filter, Davis never observed conjugation. This further proved that in order for conjugation to occur, the cells must come into physical contact. In order for cells to undergo conjugation, one cell must contain a fertility factor (F). William Hayes discovered this F factor in 1952. The F factor, which is a small auricular molecule of DNA (plasmid), controls the synthesis of F pill that connect donor and recipient cells during conjugation. These F factors are approximately 105 bagpipers in size. In bacterial conjugation, a donor cell containing the F plasmid is referred to as an F+ cell while a recipient cell that lacks the plasmid is an F- cell. When an F+ cell mates with an F- cell (conjugation), the plasmid is transferred. Both the donor and recipient cells become F+ cells and contain the F plasmid. While transferring the F+ plasmid, sometimes the plasmid is integrated into the recipients chromosome. We will write a custom essay sample on Lab Report of the Experiment of Conjugation of E. Coli specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on Lab Report of the Experiment of Conjugation of E. Coli specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on Lab Report of the Experiment of Conjugation of E. Coli specifically for you FOR ONLY $16.38 $13.9/page Hire Writer These cells are referred to as Hoff cells. Sometimes chromosomal DNA is looped out of the F plasmid, and chromosomal genes are transferred into the recipient; the recipient cells are referred to as F strains. When donor F cells mate with recipient F- cells, genomic DNA is transferred from donor to recipient. This transfer is known as seduction and the cell that receives the F plasmid from the donor is referred to as a transcontinental (Sonatas Simmons, 2006). In the experiment performed, conjugation was studied in E. Coli bacterial cells. The donor bacterial cells contained the F plasmid that had the lack+ gene integrated into it, making the cells Flag+stars. The recipient bacterial cells were F- lack-stir. The donor and recipient cells were mixed and plated onto streptomycin indicator plates. Using AGE electrophoresis, plasmid DNA was isolated and its size was determined. The plasmid was present in the donor and transcontinental cells; however, in the recipient cells the plasmid was absent. Materials and Methods:One ml of each of donor (Flag+stars) and recipient (F- lack-stir) the E. Oil bacterial strains, from the American Type Culture Collection in Rockville, Md. , was pipettes with a pitman into a sterile culture tube and incubated, without shaking, at 370 C for 90 minutes. Before plating the strains on agar plates, dilutions of the three strains of cells were prepared with LB broth. 100 Pl of 10-5 and 10-6 dilutions of donor cells were each plated onto McCracken (MAC) agar plates without streptomycin. 100 Pl of 1 0-5 dilution of donor cells and 10-5 and 10-6 recipient were also plated onto MAC plates with streptomycin. 00 Pl of 10-4 and 10-5 dilutions of the conjugation mixture cells were plated onto MAC agar with streptomycin. All seven plates were inverted and placed in a ETC incubator for about 24 hours. The bacterial colonies on each plate were counted the next day (colony counts seen in Table l). Donor colonies were picked with a sterile loop and placed into a sterile test tube containing LB broth. Recipient and transcontinental colonies were also isolated and placed into sterile test tubes containing LB broth and streptomycin. The tubes were then placed in a 37 C shaking incubator at 250 RPM overnight. After the incubation, 1. 5 ml of each of the three cultures were added to offender tubes and centrifuged at 13,200 RPM for 1 minute. An alkaline lysine procedure like that of Bromine and Doll was then performed to extract the lassie DNA with 200 Pl of alkaline SD detergent solution (Bromine Daly, 1979). After the alkaline lysine procedure was complete, the pellets were washed with a 100% ethanol and stored in a -ICC freezer. A 1% agrees gel in 0. 5 X TUBE buffer was prepared for gel electrophoresis in a gel tray. The gel tray was placed into the BIO-RADAR FIG Mapped apparatus. Loading dye was added and each sample (apron. 25 VI) was then loaded into a well. DNA markers were loaded into the first and last wells. The gel was run under program 4 for 16 hours, 180 volts forward and 120 volts reverse. When the program was knishes, the gel was placed into an tedium bromide solution to stain. After staining, the gel was gently rocked in distilled water. Using a Kodak IDEAS 290 imaging system, a picture of the gel was taken (which can be seen in Figure 1. 0). Results:During the experiment, donor (F+lack+stars) and recipient (F-lack-stir) cells were mixed and plated onto streptomycin indicator plates. Plasmid DNA was extracted from the donor and transcontinental cells and FIG electrophoresis was used to determine the plasmids size. After plating and incubating the bacterial dilutions, the cell colonies were counted. It was observed that all of the donor ells were red, all of the recipient cells were white, and the conjugation culture cells were a mix of red and white. There were too many (>300) red colonies to count on the donor 10-5 MAC agar plate and 60 red colonies on the donor 10-6 MAC agar plate. No colonies were seen on the donor 10-5 MAC agar + strep plate. There were 126 white colonies on the recipient 10-5 MAC + strep plate and 32 white colonies seen on the recipient 10-6 MAC + strep agar plate. The transcontinental 10-4 MAC + strep agar plate had 206 red and too many white colonies to count, while the transcontinental 10-5 MAC + strep agar plate had 26 De colonies and 86 white colonies (seen in Table l). Using the cell counts and their dilutions, the culture concentration was calculated. The concentration of donor cells in the 10-6 dilution was xx cells/ ml_. The concentration of recipient cells in the 10-6 dilution was 3. Axis cells/ml. The concentration of transcontinental cells in the 10-5 dilution was 2. Xx cells/ ml (Table II). The conjugation efficiency was calculated to be 16. 25% (Table Ill). Upon completion of a FIG electrophoresis, marker standards were used to determine the plasmid size and the distance traveled. The size and mobility f the bands in Marker II (Figure 1. 0) were measured and a standard curve was generated ( Figure 2. 0). This curve was then used to determine the plasmid size present in the donor and transcontinental cells. The plasmid was not present in the recipient cells. ) The plasmid traveled 14. 5 mm and was approximately 101 kilobytes long. Discussion:After plating the donor cells onto MAC plates that did not contain the streptomycin antibiotic, red colonies grew. This result is plausible because the donor cells contained the lack Oberon, which codes for enzymes that can utilize lactose as food. Cells containing this Oberon can grow on MAC plates because the plates contain lactose sugar. These two plates were then compared to the donor plate that contained the streptomycin antibiotic. No colonies grew on the streptomycin plate. This is because the donor cells did not contain the gene for streptomycin resistance. After plating the recipient cells onto MAC+strep plates, white colonies grew. This result is seen because the recipient cells lack the lack Oberon. These cells cannot utilize lactose as a food source. Also, the recipient cells were able to grow in the presence of streptomycin because they contained gene for resistance to the antibiotic. On the plates containing MAC+strep and 10-5 transcontinental cells, there were 26 red cells present. Ideally, because the cells were too dilute for conjugation to be seen, there should have been no red cells present. On the plates containing MAC+strep and 10-4 transcontinental cells, both red and white colonies were observed. The white colonies were recipient cells and the red were transcontinental. It can be determined that the red cells were the transcontinental because previously, red cells (which indicate donor cells) were not able to grow on plates containing streptomycin. Because they ere present on streptomycin plate, the cells must have undergone conjugation. After isolating the plasmids and running them on a FIG electrophoresis, it was observed that the plasmid was only present in the donor and transcontinental cells. This occurred because only the donor cells contained the plasmid. Because donor cells were not present in the recipient cells, no conjugation could occur; therefore, no plasmid would be seen in the recipient lane on the gel. The size of the F plasmid was determined by measuring the distance the plasmid traveled in the gel, and comparing it to a known marker (Marker II). The size of the F plasmid as determined to be approximately 97 kilobytes long. This was compared to the literature value, of approximately 100 kilobytes (Sealing, Paulson, and Cooper, 1991 Because the plasmid size is very close to the literature value, it can be concluded that the DNA plasmid was successfully isolated from the donor to the transcontinental cells. Genomic DNA was not transferred and no Hoff strains were formed. The conjugation efficiency was calculated and found to be 16. 25% on the transcontinental plates, meaning for every 100 cells on the plate, 16. 5 were transcontinental. A 16. 25% conjugation efficiency is a reasonable value. The value seen could be due to the fact that even though a donor cell contains the F plasmid, the plasmid is not always transferred into every single recipient cell. If this were the case, a conjugation efficiency of 100% would be seen every time. Comparing this value to an efficiency value seen in the literature of 94%, the value is a bit low (Kiang et al. , 2000). To increase the conjugation efficiency, the mixed donor and recipient cells could be left to sit for a longer period of time.

An In-Depth Guide to Yeats The Second Coming

An In-Depth Guide to Yeats The Second Coming William Butler Yeats wrote â€Å"The Second Coming† in 1919, soon after the end of World War I, known at the time as â€Å"The Great War† because it was the biggest war yet fought and â€Å"The War to End All Wars† because it was so horrific that its participants dearly hoped it would be the last war. It was also not long since the Easter Rising in Ireland, a rebellion that was brutally suppressed that was the topic of Yeats’ earlier poem Easter 1916, and the Russian Revolution of 1917, which overthrew the long rule of the czars and was accompanied by its full share of lingering chaos. It’s no wonder the poet’s words convey his sense that the world he knew was coming to an end. â€Å"The Second Coming,† of course, refers to the Christian prophecy in the Bible’s Book of Revelation that Jesus will return to reign over Earth in the end times. But Yeats had his own mystical view of the history and future end of the world, embodied in his image of the â€Å"gyres,† cone-shaped spirals that intersect so that each gyre’s narrowest point is contained inside the widest part of the other. The gyres represent different elemental forces in historical cycles or different strains in the development of an individual human psyche, each beginning in the purity of a concentrated point and dissipating/degenerating into chaos (or vice versa) and his poem describes an apocalypse very different from the Christian vision of the end of the world. The Second Coming To better discuss the piece on hand, lets refresh ourselves by rereading this classic piece: Turning and turning in the widening gyreThe falcon cannot hear the falconer;Things fall apart; the centre cannot hold;Mere anarchy is loosed upon the world,The blood-dimmed tide is loosed, and everywhereThe ceremony of innocence is drowned;The best lack all conviction, while the worstAre full of passionate intensity.Surely some revelation is at hand;Surely the Second Coming is at hand.The Second Coming! Hardly are those words outWhen a vast image out of  Spiritus MundiTroubles my sight: somewhere in sands of the desertA shape with lion body and the head of a man,A gaze blank and pitiless as the sun,Is moving its slow thighs, while all about itReel shadows of the indignant desert birds.The darkness drops again; but now I knowThat twenty centuries of stony sleepWere vexed to nightmare by a rocking cradle,And what rough beast, its hour come round at last,Slouches towards Bethlehem to be born? Notes on Form The underlying metric pattern of â€Å"The Second Coming† is iambic pentameter, that mainstay of English poetry from Shakespeare onward, in which each line is made up of five iambic feet - da DUM / da DUM / da DUM / da DUM / da DUM. But this fundamental meter is not immediately evident in Yeats’ poem because the first line of each section - its difficult to call them stanzas because there are only two and they are nowhere near the same length or pattern - begins with an emphatic trochee and then moves into a very irregular, but nonetheless incantatory rhythm of mostly iambs: TURN ing / and TURN / ing in / the WIDE / ning GYRESURE ly / some RE / ve LA / tion IS / at HAND The poem is sprinkled with variant feet, many of them like the third foot in the first line above, pyrrhic (or unstressed) feet, that enhance and emphasize the stresses that follow them. And the last line repeats the strange pattern of the first lines of the section, beginning with a bang, the trochee, followed by the tripping of unstressed syllables as the second foot is turned around into an iamb: SLOU ches / toward BETH / le HEM / to be / BORN There are no end-rhymes, not many rhymes at all, in fact, though there are many echoes and repetitions: Turning and turning...The falcon ... the falconerSurely ... at handSurely the Second Coming ... at handThe Second Coming! Altogether, the effect of all this irregularity of form and emphasis combined with the incantatory repetitions creates the impression that â€Å"The Second Coming† is not so much a made thing, a written poem, as it is a recorded hallucination, a dream captured. Notes on Content The first stanza of â€Å"The Second Coming† is a powerful description of an apocalypse, opening with the indelible image of the falcon circling ever higher, in ever-widening spirals, so far that â€Å"The falcon cannot hear the falconer.† The centrifugal impetus described by those circles in the air tends to chaos and disintegration - â€Å"Things fall apart; the centre cannot hold † - and more than chaos and disintegration, to war - â€Å"The blood-dimmed tide† - to fundamental doubt - â€Å"The best lack all conviction† - and to the rule of misguided evil - The worst / Are full of passionate intensity.† The centrifugal impetus of those widening circles in the air, however, is no parallel to the Big Bang theory of the universe, in which everything speeding away from everything else finally dissipates into nothingness. In Yeats’ mystical/philosophical theory of the world, in the scheme he outlined in his book A Vision, the gyres are intersecting cones, one widening out while the other focuses into a single point. History is not a one-way trip into chaos, and the passage between the gyres not the end of the world altogether, but a transition to a new world -   or to another dimension. The second section of the poem offers a glimpse into the nature of that next, new world: It is a sphinx - â€Å"a vast image out of Spiritus Mundi... / A shape with lion body and the head of a man† - therefore it is not only a myth combining elements of our known world in new and unknown ways, but also a fundamental mystery, and fundamentally alien - â€Å"A gaze blank and pitiless as the sun.† It does not answer the questions posed by the outgoing domain - therefore the desert birds disturbed by its rising, representing the inhabitants of the existing world, the emblems of the old paradigm, are â€Å"indignant.† It poses its own new questions, and so Yeats must end his poem with the mystery, his question: â€Å"what rough beast, its hour come round at last, / Slouches towards Bethlehem to be born?† It has been said that the essence of great poems is their mystery, and that is certainly true of â€Å"The Second Coming.† It is a mystery, it describes a mystery, it offers distinct and resonant images, but it also opens itself to infinite layers of interpretation. Commentary and Quotations â€Å"The Second Coming† has resonated in cultures all over the world since its first publication, and many writers have alluded to it in their own work. A wonderful visual demonstration of this fact is online at Fu Jen University:  a rebus of the poem with its words represented by the covers of the many books that quote them in their titles.

English Essay Example | Topics and Well Written Essays - 1000 words - 1

English - Essay Example However, such setbacks can be prevented in terms of using medical practices that are safe. Therefore, Oliver Sacks his 1995 book of essays An Anthropology on Mars expounds on seven case studies that important for discussion. For example, by making analysis of conditions and syndromes of his patients and former colleagues, he allows readers to understand several issues (Sacks 145). These include the study on the workings of the brain and the body system and their relation with the diseases that affect most of his subjects. In other words, the author intends to make the audience understand the connection between the concepts of normality and handicap conditions in the life. The writer’s method of investigation is called neurological analysis because he is a trained neurologist. Therefore, he decides to treat his subjects individually by using case studies. Similarly, the decision to approach the cases at an individual level allows the author to understand the neurological proble ms each of the persons being investigated. Alternatively, the use of one person at a time in a case study allows for closer scrutiny and analysis in terms of the repercussions of existence in a different environment. In the same scope, the ability to analyze at a time permits the readers to compare and contrast the seven cases studies that are written in the form of essays by the author in his book (Sacks 124). This is through identifying the problems discussed and the resulting consequences of each patient in his/her unique world. Furthermore, the decision to approach the investigation in the form of case studies is making it easy for alternations and adjustments especially if there is a discovery in the scientific world. Consequently, that suggests that the cases in the book are treated independently for analysis especially when a critical addition is to be made. On the other hand, the author’s approach allows for more time to collect data and information that may suit diff erent case studies at any time. Therefore, there several ways that the writer discovers the individuals discussed in the essays. For example, the case studies involve his former patients who had visited his office and explained their ailments. The people studied in the essays also involve former colleagues and patients thus understanding their neurological conditions and superseding ailment. This case is exemplified in the Case of the Colorblind Painter who finds that his perception of colors is lost after a horrible accident. Similarly, from the comprehension of the working of visions, the author expounds on the working of the brain and how it affects optical nerves and other related reflexes (Sacks 134). On the other hand, another method to discover individuals is through understanding of some medical conditions that affect citizens as noted in A Surgeon’ Life concerning his colleague. In the case analysis, Sacks explains the symptoms of Tourrette’s Syndrome and the tactics of adjustment when infected. Alternatively, the writer makes startling discoveries about individuals based on the debilitating conditions constantly mentioned in the book that affect most citizens in American and around the world. This suggests the main intention of discovery is to bring the fore the issues of humanity and the best ways having them treated. There is an interesting matter concerning whether people the author studies are either patients or subjects. I think they are both patients and subjects in the context of the neurological study by the author. First, they are patients because there are several diagnoses concerning their conditions and syndromes. For instance, the case studies entail the struggle of each person with a condition that is both painful and hard to live in terms of

Legalization of Marijuana in the USA Research Paper

Legalization of Marijuana in the USA - Research Paper Example Firstly, it is the drug’s therapeutic effect and a range of implications that make it so valuable. Using cannabis for medical purposes is nothing new. Indeed, the first written references of its medical usage date back more than five thousand years ago. THC in marijuana helps to relieve pain, reduce nausea and promote appetite. In 2010, the Congressional Research Service report indicated that marijuana has positive effects on patients with chronic diseases. In Michigan, for instance, cannabis is used in the treatment of glaucoma and hepatitis C, while in Rhode Island physicians prescribe it for patients with HIV/AIDS, multiple sclerosis, and cancer. Medical marijuana is highly applicable to relieving various disease-related disorders such as muscle spasticity, nausea, anxiety, and chronic pain. Moreover, marijuana is not as addictive as one might believe. In fact, only 9% of marijuana users become addicted, compared to 15% alcohol drinkers and 32% smokers (Welsh). Secondly, ke eping marijuana illegal is expensive. It is hard to believe, but current marijuana policies cost taxpayers as much as 42 billion dollars per year (Hardy). For instance, Utah laws state that possession of an ounce of cannabis and less can lead to six months in jail. The laws also enforce taxpayers to cover expenses for every individual who is imprisoned for the use, possession, or distribution of marijuana. As it has been estimated in a Harvard study, nearly 7 billion dollars are spent annually on catching marijuana offenders (Miron). On the contrary, if cannabis were legalized and taxed like other commercial goods, the federal government would receive huge amounts of money that then could be used for other important social needs. Overall, the tax revenues drawn from marijuana would exceed 8 billion dollars annually if taxed like alcohol or tobacco (Fairchild). Furthermore, legalization of marijuana would boost the emergence of a highly lucrative industry and create job opportunities .

The Construction Industry Development Board Construction Essay

The Construction Industry Development Board Construction Essay In Malaysia Construction affects a lot of consequence such as noise pollution, dangerous and sometime there is unhygienic to the human being and the environment. From the statistic show, construction is a one of the five most dangerous of work in Malaysia. Construction workers are two to three times more likely to die on the job than workers in other industries while the risk of serious injury is almost three times higher. The industry has not been unresponsive to this state of affairs, recognizing that consistent excellence in worker safety requires a concerted effort on the part of everyone engaged in construction related activities. The facts show that construction is indeed a dangerous industry. The tragic incidents showed that the construction industry only paid lip service to safety. Department of Occupational Health and Safety (DOSH) director-general (Abu Bakar Che Man,2006 ) said that at least 30% of construction sites have safety ratings of unsatisfactory or lower. The number of fatality and permanent disablement cases due to accident at the Malaysia construction sites is one of the highest as compared to the other sector. Construction accident that hazardous worker life. The accidents happen may cause physical injuries or health illness in long term. The Social Security Organisation (SOCSO) paid out RM1.6bil in compensation to 52,639 workers who were involved in accidents at the workplace and while travelling to. If we still ignore this problem, what is the Consequence? For sure, the safety managing is necessary. In this dissertation, we will find out the government agencies such as Construction Industry Development Board Malaysia (CIDB), National Institute of Occupational Safety and Health (NIOSH), Department of Occupational Safety and Health (DOSH) and Occu pational Safety and Health Administration (OSHA) in enforcing on the construction safety and health. 1.2 Research Background Unsatisfactory safety record of the construction industry has always been emphasize by all social circles because safety management always being ignore part and is not achieve a good implemented systematically in the construction industry in Malaysia. Safety at workplace is an issue affecting all businesses since most companies do not feel that it is vital to the success and are afraid of possibility of prosecution. Government safety and health agencies has imposed a lot of comprehensive safety regulations in the practice of the construction industry but from the level of awareness and practicability of it are generally lower than expected over the period from the announced the safety regulation. According to Abdul Ghani Khalid Construction Site Injuries: The case Of Malaysia, good safety programs would certainly help in reducing injuries at the construction site. It will also minimise construction costs, increase productivity and profitability and more importantly it could save live s of workers (khalid, 1996). Thus, these will consequently contribute positively to the construction industry and nation as a whole. Government agencies such as DOSH, OSHA,NIOSH and CIDB has give their effort to enforcement safety and health in construction sites. However, the efficiency on enforcing is fail to achieve if without the cooperation of the construction site staff and worker. The cooperation by the site staff is important to reduce the accident at construction site and good management also provide a safer working site. The player of the construction should take the responsibilities to the construction safety and health toward from a construction industry that is more stable and sustainable in future. 1.3 Problem Statement Construction is a one of five most dangerous of work in Malaysia. In 2011, the Social Security Organisation (SOCSO) paid out RM1.6bil in compensation to 52,639 workers who were involved in accidents at the workplace and while travelling to. Its increase on 15.63 per cent more than the total amount of compensation to workers involved in accident as compared to 2010 when the amount paid RM1.35bil. In this case, government lunched many program and prevention to minimize and reduce the safety problem in the construction industry. CIDB, NIOSH,DOSH and OSHA has play their role and enforced on the safety and health to prevent and decrease the accident happen in the construction site. However, the enforcement is not perfectly preventing of injuries and fatality in the construction site, by the way its fail to solve the problem because there is a lot barrier when enforcing the rule and regulation by those agencies at last cant fully work by the enforcement. This research is to identity the construction safety problem why many of enforcement or programmes are initiated by government agencies still could not reduce effectively the fatalities and injury rate in the construction industry. 1.4 Research Aims The researchers aim for conducting this research is to analyse the government agencies on the improvement safety in the construction industry and to figure out the difficult and barrier to enforcing by government agencies in the Malaysia construction industry. 1.5 Research Objective The objective of this dissertation is to :- 1.4.1 To identify the rules and regulation enforced by government agencies 1.4.2 To investigate The effectiveness of the implementation and the difficulties during the enforcement of construction safety. 1.4.3 Recommendation to improve the effectiveness of the implementation based on result by questionnaire and barrier of the enforcement of construction safety 1.6 Scope of Study This study will identify the role of government safety and health agencies and also observation worker in the construction site to figure out any improvement can be achieve by the safety and health. Program enforcing by government safety and health agencies will be discussed and the data regarding the efficiency of safety and health will be attached such as accident rate and fatalities in construction. In order to achieve the research objective, questionnaire among construction site staff around peninsular Malaysia will be analyse to acquire the result for this research. The construction site worker such as Contactor, Site Manager, Site Supervisor, Site Engineer, Site QS, and site Worker. 1.7 Research Methodology 1.7.1 Stage 1: Literature review Literature review is an integral part as the preliminary stage of information collection to obtain extra knowledge and understanding of the research. Information and data related collected according the objective of this dissertation normally from internet, book, journal, magazine and article which available in college library. Statistic regarding the construction accident and fatalities will be analysed to find out the barriers of the enforcement. This stage enable to identify the problem and to figure out the solution to solve it in a better way 1.7.2 Stage 2: Data Collection by Questionnaire A set of questionnaire will be prepared to obtain the construction site staff opinion about the safety and health on construction site. Question around 40 to 50 will distribute to the construction site worker such as Contactor, Site Manager, Site Supervisor, Site Engineer , Site QS, and site Worker. The question will designed according to the research objective there is question about rule and regulation of government agencies on site staff opinion and the difficulties among construction site worker to comply with the rules and regulation enforced by government safety and health agencies 1.7.3 Stage 3: Data Analysis and Finding This stage is analysis of the questionnaire to determine the level of responds to each question within the questionnaire by counting the number of respond who answer favourably or unfavourably. The respondent come form different aspect in the construction industry and result to determine the efficiency of the safety and health enforcing by government agencies. With the result get from the questionnaire, graphical format using by SPHH will produce to explain the result with easily. 1.7.4 Stage 4: Conclusion of Research After the data analysis done, all the data will complied into the dissertation in order to amend mistake that contained in the dissertation. Conclusion and report will be carried out in this stage. 1.8 Summaries of Chapter 1.8.1 Chapter 1 Chapter 1 is to understand about the research proposal title and main focus on the sector by a short paragraph. In this stage, also stated the aim and objective of this research from the problem statement. 1.8.2 Chapter 2 This stage is to study the deeply background of the research. Literature review on textbook, journals, article, dissertation will be conduct to collect all secondary data by according to the research aim and objective. 1.8.3 Chapter 3 Chapter 3 Methodology is to study the method of data collection. How to design of the questionnaire, how to collect the data and where the data to be source will be conduct in this stage. The research methodology us important in guiding the researcher to achieve the aim and objective. 1.8.4 Chapter 4 The result get from research methodology will be analyse and discussed. Figure and table will be created to show the analysis of the result. 1.8.5 Chapter 5 This chapter presents finding for the study the will be carried out. A summary or conclusion about this research will be discussed. 1.9 Summary In chapter 1, we have in introduction about Malaysia current Construction Safety situation. The unsatisfactory of accident rate bring a barrier toward from a construction industry that is more stable and sustainable in future. Accident cannot be fully avoided, but it can be minimise with some proper prevention. Although Government have many agencies to promote and enforcing occupational safety and health in construction industry, unfortunately the occupancies safety and health is still under a unsatisfied level. In this research, we able to know some of the rule and regulation or program that enforced by government agencies and also to figure out the barrier that threaten construction safety. Chapter 2: Literature Review 2.1 Introduction Construction industry plays a significant role in the development process of Malaysia development contribute towards the economic growth generating additional demands for construction activities. Undoubtedly construction is a industry which is very active and booming industry with contributing to the Malaysia economy. However, such achievements have also contributed much towards the safety issues where statistics showed that this industry has earned the reputation of being a highly hazardous industry due to its fatality rates.( Dayang and Gloria Chai, 2011) ) Health and safety is a paramount consideration for all construction industry professionals. It impinges upon the work of the planning authorities, client, consultants, contractor- in fact anyone who works on or is in the vicinity of a construction project. Whereas in the past health and safety, management has traditionally been consider as a production site activity, it has now become a comprehensive consideration process. It ha s also become an integral part of the corporate organizational framework and structure as well as the operational management of many organizations who contribute to the construction process. Safety management is the key to ensuring that safety measures are implemented on construction site, thus Malaysia government has been announced many agencies to manage the safety and health and a lot of enforcement or program are launched on construction site.(Steve Rowlinson, 2004) In this stage, the implementation of enforcement or program initiated by the government agencies are reviewed which including the history and function of the government enforcements. The accident rate or fatalities result that simultaneously the efficiency of construction safety enforcement in construction industry. The barrier and difficulties faced by government agencies in enforcement and program launched 2.2 Construction Safety As we know, construction bring a huge contribution to country economic growth but it also bring a impact of construction safety problem threaten construction site worker such as injuries, illnesses even death. According to statistic the Social Security Organisation (Socso) paid out RM1.6bil in compensation to 52,639 workers who were involved in accidents at the workplace and while travelling to.(The Star, 2011) Variety of unexpected accidence occur, the light caused by the loss of equipment and working hours, minor injury, but severe case may ruin and death. The construction safety and health is the process of construction industrial development, which cannot be ignored. Workers are often because of lack of experience caused accidents, the proportion of the damages around 50%, it is very serious case to threated construction site safety. Other reason cause accident on construction site such as high-altitude operation, cross-liquidity operation, long operating time, labor-intensive, monotonous duplication of work and so many, those are the reason an accident occur. For many years operating in this environment, people are likely produce a depressed, irritable mood. When the operating life of job stimulus, the visual and psychological again building site impetuous color and clutter environment, the more intensified the anxiety, depression and un rest will increasing the probability of occurrence of the accident. After a survey of different regions and cultural structures of different ages construction worker, more than 85% agree, long labor in a gray color, and illumination is not enough under the operating environment, it is easy to make people feel stress, resulting in depressed the sense of fear, ideological irritability visual fatigue, physical consumption object recognition energy increases, it tends to cause the d eformation of the job action, affecting labor efficiency, while also increasing the likelihood of potential accidents. The construction safety purpose first is to avoid or reduce causalities and occupational diseases; second is to ensure the equipment tools, and materials from damage and to protect and improve labor productivity, and maintaining social and economic development; the third is to eliminate or reduce the environmental hazards of industrial pollution and living conditions of the people from destruction, and promote the interests of humanity as a whole increases. Safety of construction threaten the safety of construction site worker lives and property of the state, is no longer just the death or injury problem, but the change for real people- oriented and full attention to the construction safety and health of every worker. Worker are requires not only the protection of life, and also comfortable in the workplace and living environment, occupational diseases prevention and control of demand, which is more important part of sustainable human development. A good construction safety will increase the performance of the construction work. It also reduced the probability of accident rate in construction site efficiently and minimize the loss and damage when fatalities occur.à £Ã¢â€š ¬Ã¢â€š ¬Thence, its important to have a good safety and health management at construction site and cooperates with government agencies on enforcement of it. http://vectorise.net/logo/wp-content/uploads/2011/04/utamakan-keselamatan.png Figure 2.1 : Poster Safety first by CIDB 2.3 Government agencies in construction safety There is no hundred percent that we can get free accident in construction, but we can minimize the accident rate happen. Safety problem is a serious problem that always concern by the government. Every year around 2 billion are paid for compensation to worker injured at construction site. According to the statistic of occupational accidents by Department of Occupational Safety and Health, 99 case of construction accident occur in 2011 and 50% of the accident involve death and permanently disable. Construction industry bring a big contribute towards the economic growth but stimulatory its also bring a problem to the construction site safety. In this case, Malaysia government has been announced a lot of program or enforcement to improve the safety of construction site through government agencies to manage the program or enforcement. Unfortunately, some of the program is have some improvement but some of them are not. That is wasting time and cost or resources to having such program or enforcement. There is necessary to find out the barrier of enforcing or program therefore enable to achieve a higher level of construction safety. 2.3.1 Construction Industry Development Board (CIDB) Construction Industry Development Board (CIDB) is strategically important as a regulator body under the jurisdiction of the Ministry of Works Malaysia in Construction Industry. CIDB was officially launched in July 1994 by Act 520 of Lembaga Pembangunan Industri Pembinanaan Malaysia Act 1994. The mission of CIDB is To develop the Malaysian construction industry and increase the competitiveness of the Malaysian construction industry. The vision is CIDB to be a distinguished organization in developing world class construction industry.(Wong Sing Ling, 2011) Develop the capacity and capability of the construction industry through the enhancement of quality and productivity by placing great emphasis on professionalism, innovation and knowledge in the endeavor to improve the quality of life is the objective of the board and to achieve the objective, 11 function laid under of Law Of Malaysia Act 520 are follow: To promote and stimulate the development, improvement and expansion of the construction industry; To advise and make recommendations to the Federal Government and the State Governments on matters affecting or connected with the construction industry; To promote, stimulate and undertake research into any matter related to the construction industry; To promote, stimulate and assist in the export of service related to the construction industry; To provide consultancy and advisory services with respect to the construction industry; To promote quality assurance in the construction industry; To initiate and maintain the construction industry information systems; To encourage the standardisation and improvement of construction techniques and materials; To provide, promote, review and coordinate training programmed organized by the public and private construction training centres for skilled construction workers and construction site supervisors; To accredit and register contractors and to cancel, suspend or reinstate the registration of any registered contractor; and To accredit and certify skilled construction workers and construction site supervisors. (source: CIDB,2012) Many program and rules and regulation that have been enforcing to safety and health to minimize the accident happen in construction site by the board. Those program are result in effectiveness to minimize the accident rate in construction site such as safety and health induction and green card program. CIDB also award those well performance construction site worker called Malaysian Construction Industry Excellence Award (MCIEA) every year. 2.3.2 National Institute of Occupational Safety and Health(NIOSH) National Institute of Occupational Safety and Health was established with an endowment fund from SOCSO and from the Government an as a company limited by guarantee under the company Act 1965to promote safety and health culture at the work place in Malaysia. NIOSH was launched in 1 December 1992 and the vision of NIOSH is To be the Leading Centre of Excellence in Occupational Safety and health and the mission is To be the preferred partner in enhancing Occupational Safety and Health.( Fadhil,2010) Objective of NIOSH is to ensure a healthy working environment for all employees, course participates, contractors and visitors. A lot of contribute towards efforts in upgrading Occupational Safety and Health ( OSH) through developing curriculum and training programs for workers and employers to assist industry, commerce and other to solve problem relating to Occupational safety and Health (OSH). NIOSH also conduct primarily applied research and both short term and long term research on OSH and health issues relevant to the country and to disseminate information on research and findings on OSH and health issues in the main and other pertinent activities undertaken by or on behalf of the Institute and to be a centre of reference. (source: All about Safety, 2001) The program indicated by NIOSH such as Contractor Safety Passport to increase the knowledge and awareness about occupational safety and health in construction industry through training or short courses. Participant are receive a certificate by NIOSH after sit for an examination as certify participant is fully understand their responsibility to occupational safety and health in the industry. 2.3.3 Department of Occupational Safety and Health (DOSH) The Department of Occupational Safety and Health (DOSH) is a department under the Ministry of Human Resources. This department is renamed from the Department of Factory and Machinery in April, 1994 and it is responsible for ensuring the safety, health and welfare of people at work as well as protecting other people from the safety and health hazards arising from the activities sectors which include: Manufacturing, Mining and Quarrying, Construction, Hotels and Restaurant, Agriculture, Forestry and Fishing, Transport, Storage and Communication, Public Services and Statutory Authorities, Utilities Gas, Electricity, Water and Sanitary Services  ,Finance, Insurance, Real Estate and Business Services and Wholesale and Retail Trades. As a government agency, the department is responsible for the administration and enforcement of legislations related to occupational safety and health of the country, with a vision of becoming an organisation which leads the nation in creating a safe and healthy work culture that contributes towards enhancing the quality of working life. With the corporate values Firm, Fair and Friendly by DOSH, the objective of the Department is to prevent industrial accident and occupational diseases by achieving reducing the rates of fatalities and rate of reported accident up to 20% . Furthermore, increasing enforcement investigations, increasing promotional activities of safety and health and increasing the number of Occupational and health practitioners up to 10,000 people also is one of the objective by DOSH. In order to achieve a higher level performance of safety and health DOSH study and review the policies and legislation of occupational safety and health with enforce legislations such as Occupational Safety and Health Act 1994 and its regulations, Factories and Machinery Act 1967 and its regulations and Part of Petroleum Act 1984 (Safety Measures) and its regulations. DOSH also conduct research and technical analysis related to occupational safety and health at the workplace to carry out promotional and publicity program to employers, workers and the general public to foster and increase the awareness of occupational safety and health and become a secretariat for the National Council regarding occupational safety and health. (Amat, 2008) 2.3.4 National Council for Occupational Safety and Health (NCOSH) The National Council of Occupational Safety and Health (NCOSH) is the highest forum in the Ministry of Human Resources where tripartite discussions are held about issues related to the direction, national policy and the implementation of occupational safety and health in Malaysia. NCOSH was formed by the Ministry of Human Resources in 1995 under Section 8 of the Occupational Safety and Health Act 1994 (Act 514), as a product of the Government continual efforts to make safe and healthy work practices as a part of Malaysian culture.   Furthermore, its purpose is to improve level of awareness of occupational safety and health and to ensure the safety of all workplaces in Malaysia. To become the prime mover in steering the nation through tripartism, forming a safe and healthy work culture as well as raising Malaysians quality of life. NCOSH carries out its functions by conducting discussions, studies and investigations, and subsequently presenting suggestions to the Ministry of Human Resources on matters related to Act 514 in particular the following matters:- Changes deemed appropriate for occupational safety and health legislation Administration and enforcement of occupational safety and health legislation Open communication between management and employees regarding workers safety, health and welfare Adequate control methods for industrial chemicals in the workplace Work-related death and accident statistic analysis Issues dealing with the safety, health and welfare of woman the handicapped and other groups in the community Encouraging the development and acceptance of an industrial codes of practice related to workers safety, health and welfare Development rehabilitation plan and facilities to help those injured in the workplace. (source: OSH,2002) 2.4 Enforcement by Government Agencies in Construction Safety and Health Many of Government Agencies put their biggest afford to enforce the safety and health with a lot of program or rules are initiated on construction site. Implementation of enforcement or program initiated by the government agencies is to increase construction worker knowledge and awareness about occupational safety and health and effectively reduce the fatality or injury rate on construction site. 2.4.1 Contractor Safety Passport (CSPS) Contractor Safety Passport is initiated by NIOSH to provide some basic skill and induction about the knowledge of occupational safety and health. Participant are receive a certificate by NIOSH after sit for an examination as certify participant is fully understand their responsibility to occupational safety and health in the industry. In the past of responsible company may have its own system ascertaining which contractor should be awarded a contract of work based on a number of criteria. One such criterion is that the contractor has workers who are well-versed with the rudiments of occupational safety related to their trade. In this way, the company will be assured that the workers concerned are able to work with minimal supervision without fear of any untoward incidents occurring during the course of package to help contractors and their worker to attain minimum standard of safety. (source: NIOSH 2012) 2.4.2 CIDB Green Card CIDB Green Card initiated by CIDB since August 1997. This is an integrated program that involve the registration and accreditation of Construction Personal to enhance safety level at Construction Sites and ensure that construction workers are aware of the importance a safe and healthy working place and provides a basic knowledge on safety and health at the construction work site. This Program entails a one day Safety and Health Induction Course for construction Workers and provision of personal protective equipment consisting of a safety booklet and helmet. Green Card will be issued to participants of the one day safety and health induction course which also services as proof of registration with CIDB under the category of construction personnel.. Those Construction Personnel who have been registered with CIDB and issued the Green Card are automatically covered by a special Insurance Scheme that insures the Construction Personnel against death and accidents. This insurance scheme is the result of CIDB continuous efforts towards improving construction workers welfare as well as providing security for them and their loved ones. Since August 1997, when this scheme was launched, construction personnel have enjoyed a 24-hr worldwide coverage which is not limited to accidents at work sites alone. Objectives of the Green Card Program divided into 3-fold. First is to ensure that the construction worker is aware of the importance a safe and healthy working place. Second provide a basic knowledge on safety and health at the construction work site and third inform construction worker of the legal requirement is relation to safety and health. ( source: Green Card Program,1997) https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjx1nLQMgvWk0q3V8kZcfYIB4ZVeLJcdPbF9IOtynHOcNNBseRKA9FMiqJZNSc84FIvR5OBXtneutDxQyf6P8i9J7vZ07v1Qm10njQdq5ZgZo3aPwRovAWUjasDJj1PqpFjNS19_8-oCv4/s1600/PA280900.JPG Figure 2.2 CIDB Green Card 2.4.3 Occupational Health and Safety Committee Functions of safety and health committee is keep under review the measures taken to ensure the safety and health of persons at the place of work. According to the Law Of Malaysia Act 514, every employer shall establish a safety and health committee at the place of work in accordance with this section if there are forty or more persons employed at the place of work; or the Director General directs the establishment of such a committee at the place of work. ) The composition of a safety and health committee established under subsection (1), the election or appointment of persons to the committee, the powers of the members of the committee and any other matter relating to the establishment or procedure of the committee shall be as prescribed. Every employer shall consult the safety and health committee with a view to the making and maintenance of arrangements which will enable him and his employees to co-operate effectively in promoting and developing measures to ensure the safety and h ealth at the place of work of the employees, and in checking the effectiveness of such measures. A person who contravenes the provisions of this section shall be guilty of an offence and shall, on conviction, be liable to a fine not exceeding five thousand ringgit or to imprisonment for a term not exceeding six months or to both. (source: Act 514 OSHA 1994) 2.5 Occupational Accidents and Fatalities Rate According to the statistic of occupational accidents by Department of Occupational Safety and Health, 99 case of construction accident occur in 2011 and 51 people are death and 5 person is permanently disable in the accident, it is over 50 percent out of total accident. C:UsersKitPicturesUntitled.jpg Figure 2.3 Data of Occupational Accident 2011 provided by DOSH Compare to the result in 2010, it is slightly reduce of the amount of victim involve from 100 to 99 people. By the way it is still on a very unsatisfied level of construction occupational safety and health. Figure 2.4 Data of Occupational Accident 2010 provided by DOSH 2.6 Barrier and Difficulties in Enforcing on Safety and Health by Government Agencies Construction industry plays a significant role in the development process of Malaysia development contribute towards the economic growth generating additional demands for construction activities. Undoubtedly construction is a industry which is very active and booming industry with contributing to the Malaysia economy. However, such achievements have also contributed much towards the safety issues where statistics showed that this industry has earned the reputation of being a highly hazardous industry due to its fatality rates.( Dayang and Gloria Chai ) In order to form a construction industry that is more stable and susta

“A Soldier” by Robert Frost

Andrew Fariello Professor Didner ENC1102 MW 3:30-5:15 â€Å"A Soldier† by Robert Frost â€Å"A Soldier† by Robert Frost Robert Frost's â€Å"A Soldier† attracted my interest to some degree. As a United States military veteran of a foreign war, I significantly related to the message that Robert Frost was sending. From my own personal experiences that I have endured while fighting in Operation Iraqi Freedom conflict in Iraq, Robert Frost's words exploded imagery into my mind breaking open another dimension that typed words on a paper could not provide alone.His poem really hit home, creating a bond between the poem and myself, making â€Å"A Soldier† a wonderful poem for me to analyze. In the poem â€Å"A Soldier,† Robert Frost uses a hurled lance that will eventually rot away to symbolize a dead solider that too, will be forgotten soon. Frost is describing a soldier that has been killed by war, and has been forgotten due to the fact that the soldier is just that: a soldier, a killer that had been killed for an unimportant ugly cause. Robert Frost portrays an image that the soldier did live for a greater cause, and should be remembered for it.Robert Frost first describes a lance that has come to rest on the ground, and will soon rot away. He is that fallen lance that lies as hurled, That lies unlifted now, come dew, come rust, â€Å"He is that fallen lance that lies,† and â€Å"That lies unlifted,† tells the reader that the soldier is dead. The â€Å"fallen lance† is â€Å"He,† the soldier. â€Å"Lies unlifted† tells the reader that the soldier is dead, not uplifted as the reader would expect to have read rather than the word â€Å"unlifted. The lance, which is the soldier, will wither away just as a lance left in the ground would rust away. Then Frost tells his readers that society doesn't see the soldier's life important enough to have solved anything, and that the soldier's death didn't hav e any meaning. Before Frost tells his readers this, he first gives a hint that the soldier's death did have meaning. But still lies pointed as it ploughed the dust. If we who sight along round the world, See nothing worthy to have been its mark, It is because like men we look too near,Forgetting that as fitted to the spear, â€Å"See nothing worthy to have been its mark† symbolizes that the soldier wasn't â€Å"worthy†, or important enough to have come to lie permanently in the â€Å"dust†, or the earth. Although society couldn't see a larger picture, and no meaning behind the soldier's death, as Frost portrays as â€Å"men† who â€Å"look too near†, the soldier did, in fact, have a greater meaning to it all. Frost portrays this theory by keeping the lance, or solider â€Å"pointed as it ploughed the dust†, meaning the solider was pointed in the right direction in his life.Lastly, Frost describes the burden that is created from our weapons, but shows us that the soldier will be triumphant after his death. Our missiles always make too short an arc. They fall, they rip the grass, they intersect The curve of Earth, and striking, break their own; They make us cringe for metal-point on stone. But this we know, the obstacle that checked And tripped the body, shot the sprit on Further than target ever showed or shone. â€Å"Too short an arc†, and â€Å"they intersect†, symbolizes that our weapons are designed to come down to earth and destroy. Break their own† and â€Å"metal-point to stone† symbolizes that once the weapons do come down, they will destroy their own race and their creations. The soldier though, â€Å"tripped† and â€Å"shot the sprit on,† depicting that he is in a better place now. And the soldier is now at peace. Robert Frost used rhyme to keep the reader's interest, and implemented it very cleverly into his poem. The last word in the first line of his poem rhymes with the last word of the fourth line.And the last word of the very next line rhymes with the last word of the eighth line, which is the same interval of lines between the first and fourth lines. This pattern continues throughout the poem, and the lines that are in the middle of these intervals, also have their last words rhyming with each other. In conclusion, Robert Frost paints an image of a passed soldier that many may have been forgotten. Although only few will see the bigger picture behind the soldier's death, the soldier's sprit has moved on to a place where it can't be exposed anymore; â€Å"Further than target ever showed or shown. â€Å"

A History of Argumentative Essay Topics Technology Privacy Refuted

A History of Argumentative Essay Topics Technology Privacy Refuted If you wish to learn what an argumentative essay is, the very first thing you need to remember is that its primary objective is to convince the audience to accept your viewpoint. You might also want to incorporate a quick discussion of more research that needs to be completed in light of your work. The position is not really that Myrtle should include inside her essay. Even in case you don't like to argue with the points of different individuals, it's still true that you have to be prepared to compose an argumentative essay at any moment! In the debut, that is the very first paragraph of the essay, Myrtle will want to spell out the problem and state her position. Make sure your reader is left with an impression or something to consider in connection with your topic. The Basics of Argumentative Essay Topics Technology Privacy Even when you're writing an informative essay, it's still true that you have the jo b of attempting to convince your audience that the info is essential. It is crucial to check reviews about essay writing services in order to be confident they can deliver your task before the deadline. Whether you cannot decide on topics for argumentative essay, don't understand how to begin, or simply require a good example of a well-structured paper in a particular format, we're always prepared to help! Then you ought to endeavour to select an appropriate argumentative essay format. A number of you might be knowing an outline gives a hint about the standard of an essay. As a writer, using logos ought to be primary, should appear throughout the essay, and it's the very best approach to convince a person to adopt a specific stance on any situation. By now you're probably keen to find a superb argumentative essay outline template. Using Argumentative Essay Topics Technology Privacy Care ought to be taken that the data utilised in the essay is pertinent to the topic. It is essential that the thesis statement needs to be slimmed down as a way to stick to the guidelines of the given writing exercise. If your professor didn't provide any particular requirements, you can choose the subject by yourself. An argumentative essay example will reveal the should possess some crucial components which make it better in the practice of convincing. Moreover, a verbal argument often focuses on who's right regarding a particular issue, though a well-written, researched argu ment essay focuses on what's the correct side of a certain issue. An argumentative essay presents either side of a problem. For this reason, you shouldn't steer clear of a bit of controversy. Argumentative Essay Topics Technology Privacy You need to have the strong personal opinion on the subject you intend to discuss in your argumentative essay. To pick a topic that you aren't interested in or do not care about is a really terrible idea. It is advised to steer clear of argument essay topics on moral issues because they don't support logical discussion. A debatable topic is one which has differing viewpoints. There are invariably a broad range of opinions on the topic of technology, and here are our favourite things to consider on this issue. The very best idea is to select essay topics that actually matter to you. Besides general knowledge you have regarding the subject, you won't mind dedicating some opportunity to research, writing procedure, and other things essential for the high-quality essay. Quite frequently, the very best topic is one which you truly care about, but you also will need to get well prepared to research it. For instance, to write about the impact of video games on child development isn't a great idea if you haven't ever played video games yourself. The first thing you're likely to have to do is to pick a topic that has more than one clear side. If you're on the lookout for help, BibMe has a grammar check service you can test out. For instance, you can write about something that personally affects you or somebody you know. Argumentative Essay Topics Technology Privacy at a Glance You can also see academic essay. The essay ought to have a little bit of structure, unlike a normal essay. You can also see analytical essay. You might also see descriptive essay. If you decide to be an independent essay writer, you can expect the exact same. To construct an effective argumentative essay, you should follow several critical measures. It is crucial to remember an argumentative essay and an expository essay could be similar, but they vary greatly concerning the quantity of pre-writing and research involved. This an indication of a rather strong argumentative essay.